All Exhibitor Presentations will take place in Room 123 of the Moscone Center.
Asylum Research, an Oxford Instruments Company
Bruker Nano Surfaces
FEI Company: Presentation 1, Presentation 2, Presentation 3
Forte Bio, A Division of Pall Life Sciences
Molecular Devices, LLC: Presentation 1, Presentation 2
Nanion Technologies: Presentation 1, Presentation 2
Park Systems, Inc.
World Precision Instruments, Inc.
Wyatt Technology Corporation
Sunday, February 16
Sunday, February 16, 8:00 AM - 8:45 AM
High End Microscope Platform for Multimodal Live Cell Imaging
Ultimately, the secrets of life can only be studied in the living stage - dynamic processes have to be followed in space and time in living cells to fully understand their interplay. Successful live cell imaging experiments require minimizing the phototoxicity while the acquisition speed has to match the dynamics of the process to be studied. Especially on rare samples, extraction of the highest possible amount of data from a single experiment is needed.
The iMIC, our digital fluorescence microscope, has been optimized to meet the challenges of live cell imaging. It offers fast measurement at best sensitivity and minimal bleaching. Depending on the sample and the process to be studied, a variety of specialized microscopy techniques can be chosen to optimize the result. Fast wide-field imaging, spinning disc confocal, FRAP and FRET can be combined in one flexible setup and used on the same sample. Moreover FEIs unprecedented solution for TIRF imaging makes the iMIC an even more valuable instrument.
TIRF is the way to get superior Z resolution using affordable laser and camera technology. However, constant need for realignment and inhomogeneous excitation have been drawbacks of this technology, especially for quantitative measurements. Our motorized multi-point TIRF module, giving full control over penetration depth for different excitation wavelengths, automatically adjusted TIRF angle and a simple user interface, brings the application to a next level. To monitor even fast processes in living cells utilizing different modalities, switching between TIRF, epifluorescence or FRAP is possible within milliseconds, using our Polytrope imaging mode switch.
FEI’s proprietary confocal spinning disk design excels with superior resolution and best alignment of color channels in multi-color 3D image stacks. Transmission through the disk is enhanced by micro-mirrors, not micro-lenses. This concept allows us to achieve perfect achromatic correction in the wavelength range of 405 to 700nm.
Most imaging techniques can also be combined with our two-photon microscopy solution. Based on the renowned digital Yanus laser scanner and GaAsP photomultipliers with large sensitive surface, the two-photon implementation yields very large fields of view with perfect resolution corner to corner.
Meike Pedersen, Product Marketing Manager at FEI Munich GmbH
Tilman Franke, Product Marketing Manager, FEI Munich GmbH
Gregor Heiss, Product Marketing Engineer at FEI Munich GmbH
Forte Bio, A Division of Pall Life Sciences
Sunday, February 16, 9:00 AM - 10:30 AM
Developing Assays for Kinetic Characterization on the BLItz System
The BLItz system is a simple-to-use, affordable label-free assay system capable of delivering rich insight into real-time binding interactions of antibodies and proteins. This workshop will present several case studies of how the BLItz system is being used to validate biophysical models.
As part of the workshop, learn tips and tricks for developing a kinetics assay on the BLItz system and take the opportunity to perform a hands-on demo of the system.
Presenter: Renee Tobias, Applications Scientist, Pall ForteBio
Molecular Devices, LLC
Sunday, February 16, 11:00 AM - 12:30 PM
Investigating Use-Dependent Inhibition of Ion Channels on Automated Electrophysiology Systems including the IonWorks Barracuda® Instrument and the IonFluxTM Benchtop Reader
Use- dependent inhibition of ion channels by potential drug candidates is an important aspect to investigate for many drug classes. Use-dependent drugs specifically target ion channels in cells that are more electrically active. For example, a drug targeting pain that is more potent to Na+ channels in neurons actively firing action potentials is a better drug candidate. Data will be presented to demonstrate the ability of automated electrophysiology systems to study the use-dependence block of Na+ channel targets. Tetracaine, lidocaine, and TTX exhibit very different behavior in terms of their use- dependent blockage. We will demonstrate the ability of the instrumentation to deliver complex voltage protocols and generate long assay windows which are required for these studies. Pulse trains delivered at 10Hz are used to measure the blockade of current. Data from a separate study will also be presented that demonstrate blockage and enhancement of NaV1.5 currents by various peptide toxins. Both sets of experiments demonstrate stable assay windows with uniform currents for 30 minutes and longer during the delivery of periodic pulse trains.
Presenter: James Costantin, Product Marketing Manager, Automated Electrophysiology, Molecular Devices, LLC
Sunday, February 16, 1:00 PM - 2:30 PM
New Advances in Fitting Kinetic and Equilibrium Data by Simulation
Fitting kinetic data based upon numerical integration of rate equations offers many advantages over conventional fitting of data based upon equations derived from simple models. Fitting by simulation is the most rigorous and eliminates the need to derive equations; however, it also requires and understanding of the kinetics and critical thought to avoid overly complex models.
In this presentation, Dr. Johnson will show how global fitting of kinetic data can be accomplished with ease using the fast, dynamic simulation in KinTek Explorer software, overcoming the all-to-common errors in conventional fitting. Moreover, data are fit to derive rate constants directly defining steps in a model. New advances in the software allow fitting kinetic data from single molecule experiments and families of curves can be fit simultaneous to define voltage-dependent rate constants or data from Temperature-jump or Pressure-jump experiments. In addition, equilibrium titration data can be fit using a unique endpoint simulation method, and time-resolved spectra can be fit using singular value decomposition (SVD). Moreover, all experiments can be fit simultaneously.
Presenters: Kenneth A. Johnson, President, KinTek Corporation
Roger Williams Professor of Biochemistry, University of Texas at Austin
Sunday, February 16, 3:00 PM - 4:30 PM
Development of Automation and Nanofluidics to Extend Applications of Atomic Force Microscopy
In an effort to extend the range of atomic force microscope (AFM) applications, we have developed automation routines for nanomechanical analysis of large uneven samples and incorporated nanofluidics for nanomanipulation experiments.
We will present details of a method that has been developed to compensate for the Z-range limitation and to automate the data collection over large sample areas. To compensate for large surface corrugations on biologically relevant samples, customized hardware and software algorithms for automated leveling have been developed and implemented. This method consists of a patented vertical alignment system, which is activated whenever the Z piezo reaches its limit (i.e., max. extension or max. retraction). This method allows for AFM investigation to proceed uninterrupted and error-free over corrugated surfaces.
FluidFM combines the positional accuracy and force sensitivity of AFM with the unique possibilities of nanofluidics to provide a whole new level of control and possibilities in nanomanipulations and analysis. The FluidFM system includes a fully integrated AFM, pressure controller and hollow microfabricated cantilevers. The integrative nature of its touchscreen-based control software brings together optical, force, pressure, and position control in one place. The entire system is easy to use and allows objects and experimental settings to be manipulated via on-screen interactions. Moving a sample or indicating measurement positions has never been more intuitive. Details of several different applications of FluidFM in cell biology will be presented including pick and place of single cells, single cell force spectroscopy, cellular injection and micropatterning under liquids.
Marko Loparic, Research Associate, Biozentrum and the Swiss Nanoscience Institute, University of Basel
Saju Nettikadan, General Manager, Nanosurf Inc
Asylum Research, an Oxford Instruments Company
Sunday, February 16, 5:00 PM - 6:30 PM
New blueDrive™ Photothermal Excitation for Superior AFM Tapping Mode Imaging
Asylum Research, an Oxford Instruments company, will introduce its new blueDrive Photothermal Excitation capabilities exclusively available on Cypher™, the highest resolution fast scanning AFM. blueDrive significantly enhances the performance of tapping mode imaging with more simple, stable and quantitative operation, and providing extremely clean tunes in both air and water. Typically, a piezoacoustic excitation has been used to drive the cantilever oscillation. Though piezo drive is favored for design simplicity, the response of the cantilever is often far from ideal, causing users to spend countless time selecting a clean cantilever tune. Asylum’s blueDrive excitation mechanism produces an almost perfect response by directly exciting the cantilever photothermally with a blue laser. blueDrive is ideal for high resolution imaging of biological samples in fluid including proteins, lipids and nucleic acids, as well as force measurements and nanomechanics. In this presentation, we will explain how blueDrive works, how it achieves simple cantilever tunes, and show real world results for biophysics applications.
Presenter: Nick Geisse, Applications Scientist, Asylum Research, an Oxford Instruments Company
Sunday, February 16, 7:00 PM - 8:30 PM
Cryo-TEM: A New Era for 3D Structural Analysis of Protein Complexes
A new frontier exists in unraveling interactive biological and biochemical processes and pathways at the macromolecular level. Of critical importance is the three-dimensional visualization of macromolecular structures and molecular machines in their native functional state. Three techniques play a major role in orchestrating this.
Nuclear magnetic resonance (NMR) has the capability to study specific protein domains or fragments and their functional role in protein folding and dynamics and in ligand binding whereas X-Ray crystallography (XRD) allows visualizing high-resolution but more static 3D structures of apo and liganded proteins, mainly in a monomeric or dimeric state after crystallization. To unravel more physiologically relevant situations however, it is essential to visualize multimeric complexes in their tertiary and quaternary state and their interaction with other complexes. By performing typical cryo-TEM applications like single particle analysis or tomography, this can be achieved. In this so-called translational methodology, cryo-TEM thus provides complementary information to NMR and XRD that can be crucial for drug discovery, e.g. in terms of a better understanding of the mechanism of action inferred from the EM structure of the physiologically relevant complex. This will eventually contribute to answer real biologically as well as medically relevant questions.
Latest developments in the cryo-TEM workflow have brought the three major structural biology technologies closer together. Now, finally, a continuum has been reached on all important aspects with regards to resolution and macromolecular scales which allows for the full deployment of the combination of these technologies.
Here, we will illustrate the historical context of these technologies with respect to one another and show how latest developments have reached the critical requirements to fully unleash the power of structural biology in not just answering fundamental questions, but actually contribute to curing diseases and improving health. Also, we will discuss the future of structural biology based on the latest developments of the FEI workflow and its components.
Monday, February 17
Monday, February 17, 8:00 AM - 8:45 AM
Making Correlative Experiments Easier
Fluorescence microscopy excels at labeling components of the cellular machinery with unmatched sensitivity and specificity; however, it lacks any contextual information. Providing full morphological information at the ultra-structural level is the strength of electron microscopy. If the very same sample is imaged by fluorescence and electron microscopy it is possible to merge dynamics, label specificity and nanometer resolution. Although a powerful approach, it is challenging and low-throughput.
FEI has recently introduced new solutions to overcome these experimental hurdles: CorrSight, a dedicated light microscopy system offering CLEM-specific functionality and automation of important workflow steps; MAPS, a software tool bridging the modalities to increase ease of use; and iCorr, a light microscope module integrated into the Tecnai family of transmission electron microscopes. These tools address different correlative workflows helping to optimize efficiency and data quality across the full range of CLEM experiments.
CorrSight is an innovative light microscope providing unprecedent solutions to optimal sample support for different workflows in correlative light and electron microscopy. One of its strengths is the possibility to perform live cell imaging, event-triggered fixation and subsequent processing of the sample for electron microscopy. On top of it a dedicated cryo stage allows contamination-free imaging of vitrified samples with the highest resolution.
MAPS also allows for correlation of light microscopy data captured on any light microscope with EM data acquisition on the full range of FEI SEMs / SDBs. To allow utmost flexibility in the choice of the light microscope, there is absolutely no dependence on any special hardware – correlation is carried out only on image data. Thus, existing or specialized light microscopy setups can be easily used for CLEM experiments and correlation can be carried out on any feature visible in both modalities. When coupled with CorrSight the correlation is possible without manual intervention.
In order to perform correlative experiments between LM and TEM imaging FEI has developed an integrated light and electron microscope: iCorr. This tool provides fast and effortless navigation for correlative experiments.
Alex de Marco, Product Marketing Manager, FEI Munich GmbH
Gregor Heiss,Product Marketing Engineer, FEI Munich GmbH
Liesbeth Hekking, Applications Development Engineer, FEI Company
Matthias Langhorst, Segment Director Cell Biology Solutions, FEI Company
Park Systems, Inc.
Monday, February 17, 9:00 AM - 10:30 AM
New Door to Live Single Cell Research
Atomic Force Microscopy (AFM) is a powerful measurement technique for nanoscale science. AFM is able to provide high-resolution imaging of biological structures below the optical limit, as well as the monitoring of the dynamics in biological systems and processes under physiological conditions; however, certain limitations for AFM still exist in the field of bio-applications. In recent times, the development of another kind of scanning probe microscopy (SPM) technique, scanning ion conductance microscopy (SICM), has overcome these limitations and enabled noninvasive, nanoscale investigation of live cells. SICM applications include imaging of cell topography, monitoring of live cell dynamics, mechanical stimulation of live cells, surface patterning, and so forth.
We at Park Systems have developed AFM for advanced nanoscale metrology, which separates the z-scanner from the x-y scanner. An independent z-scanner also provides an excellent platform for developing other SPM techniques such as SICM. In addition, the platform which separates the z-scanner from x-y scanner, enables us to easily switch between an AFM and an SICM z-scanner to apply both techniques without moving samples. The common glass micropipette is used in SICM as the sensitive probe, instead of a silicon-based stylus, and can glide over live cells while maintaining an absolute non-contact imaging mode. Its electrochemical current feedback system further enhances biological sample imaging. Combining confocal fluorescence data to the SICM 3D data, using an image overlay feature, provides even more data about structure of cells as related to their membranes. These advances of convergence in instrumentation will be utilized in various kinds of biomedical research and become a new driving force for biophysics and nanobioscience.
Sangjoon Cho, Senior Director of Research & Development, Park Systems, Inc.
Monday, February 17, 11:00 AM - 12:30 PM
Workshop on Automated Patch Clamp: From Single Channels, Primary Cells, Action Potentials to 384 giga-seal Recordings in a Parallel HTS Format
The Port-a-Patch recently turned 10 years old, and is going stronger than ever. It’s still the smallest patch clamp rig in the world, and makes patch clamp recordings accessible to anyone spending a couple of hours with it. Giga-seal recordings and the excellent voltage-clamp of the cellular membrane ensure high quality data, and the Port-a-Patch add-ons allow unprecedented experimental freedom, including temperature control, internal perfusion, automated action potential recordings, and recordings from primary and stem cell-derived cells. Recently, the Port-a-Patch technology was scaled up to eight simultaneous recordings (Patchliner), maintaining the same data quality and experimental possibilities, and now we did it again: 384 Port-a-Patches have been shrunk to fit inside a shoebox – called the Patch Engine (PE). Two Patch Engines can be integrated per SyncroPatch 384PE platform, allowing for patch clamp-based ion channel HTS from up to 768 cells in parallel, and we will tell you more about it during this workshop.
Another topic for the workshop is the bilayer-reconstitution of ion channels and nanopores, efficiently investigated using the Orbit 16, a parallel device for formation of and recordings for up to 16 artificial bilayers at once. Using Micro Electrode Cavity Array (MECA, Ionera), a 4 x 4 array of circular micro-cavities in a highly inert polymer, the bilayer is automatically formed by remotely actuated painting (Ionera- SPREAD).
Welcome to our workshop and learn from live, hands-on experiments on the Port-a-Patch and Orbit 16, and let us show you how to scale up your ion channel screening project to HTS-standards! Spaces are limited so reserve yours by sending an email to email@example.com.
Presenters: Niels Fertig, CEO, Nanion Technologies
Andrea Brüggemann, CSO, Nanion Technologies
Gerhard Baaken, Ionera
World Precision Instruments
Monday, February 17, 1:00 PM - 2:30 PM
Applications in Biophysics Utilizing World Precision Instrument’s (WPI) New Biofluorometer
Introduction to WPI’s New Biofluorometer with high-power LED modules. Potential applications and experimental design will be discussed in the field of Biophysics, including integration with Muscle Physiology experiments and microscopy systems for general fluorescence applications.
Presenter: Mathias Belz, Director of Optics, World Precision Instruments, Inc.
Bruker Nano Surfaces
Monday, February 17, 3:00 PM - 4:30 PM
Atomic Force Microscopy for Biological Research
Physical properties including structures such as shape/size and mechanics such as strength/stiffness/ interaction forces play crucial roles in biological processes. Quantification of this at various length scales is necessary because of the heterogeneous/complex nature of biologics. Atomic force microscopy (AFM) is a unique research tool because of its abilities to perform measurements with both high spatial and force resolution in fluid under physiological conditions. In this tutorial, Bruker will present theories behind AFM, bio-applications in high-speed AFM, and practical guides to quantitative mechanical measurements and analysis of biological samples ranging from a single membrane protein to a single cell. While the key experiments presented will encompass research in microbiology/pain mediation/cancer, the methodology has also been employed in other disciplines including pathogenesis/stem cell differentiation/cell signaling and more.
Presenter: Senli Guo, Application Scientist, Bruker NanoSurfaces
Monday, February 17, 5:00 PM - 6:30 PM
HEKA Electrophysiology Update
For over 40 years, HEKA Elektronik has provided innovative products, expert tech support and unmatched service to their customers. HEKA's commitment to technological innovation is reflected by consistent updating of both hardware and software. While yesterday's gold standards try to keep pace with the latest research techniques, HEKA takes the lead.
By popular demand, HEKA is hosting a series of user meetings with tutorial presentations. On one hand, some of the new products will be showcased to the experienced user and, on the other hand, step-by-step guidance is provided to the researcher who is new to the field. Registration is available online through the HEKA Events Page (http://server.hekahome.de/scripts/events.php), or by email to firstname.lastname@example.org. The number of available spaces, food and drink are limited, and registrations are accepted on a first-come-first-served basis.
Who should attend?
- Scientists with experience in patch clamp electrophysiology and related scientific techniques
- Researchers who want to become more efficient in the use of electrophysiology acquisition and analysis software
- PostDocs and graduate students who want to learn more about electrophysiology techniques
Hubert Affolter, Senior Software Architect, HEKA Elektronik
Jan Dolzer, Vice President Sales & Marketing, HEKA Elektronik Global
Telly Galiatsatos, General Manager, HEKA Instruments
Tuesday, February 18
Wyatt Technology Corporation
Tuesday, February 18, 9:00 AM - 10:30 AM
Essential Biophysical CharacterizationTM
: Molar Mass, Size, Charge and Interactions – The Light Scattering Toolbox for Biomolecules and Nanoparticles
Wyatt Technology provides the essential tools for characterization of biomacromolecules in solution, including peptides, proteins and oligonucleotides as well as bionanoparticles such as exosomes and VLP’s. This presentation describes the light scattering instrumentation and techniques used in these analyses: coupled to liquid chromatographic separations for absolute molar mass and size distributions (SEC/FFF-MALS); microtiter plate-based, high throughput screening of size, aggregation and interactions (DLS); and the label-free, immobilization-free analysis of biomolecular interactions for affinity and absolute molecular stoichiometry (CG-MALS). A variety of examples illustrate the unique capabilities of these light scattering measurements in biophysics.Presenters:
Chris Broomell, Wyatt Technology Corporation
Tuesday, February 18, 11:00 AM - 12:30 PM
SURFE2R - Catch the Wave for Transporters
Precise Measurements of Membrane Transporter Protein Activity
Ion transporters and pumps play an important role within general metabolism and information processing of organisms. Dysfunction and -regulation of transporter proteins are related to diseases like obesity, diabetes, and hypertension, and CNS disorders such as epilepsy and depression. Hence, ion transporters have become potential targets within the drug development treating disease-related abnormalities. At present, labeling technologies and conventional patch clamp are commonly used for ion transporter screening. However, radioactive and fluorescence-based assays have limited sensitivity, and because of the limited molecule turnover per seconds of transporters and pumps compared to ion channels, the direct electrophysiological measurement of protein transporters and pumps activity is extremely challenging.
Here, we present the SURFE2R technology – an easy-to-handle, highly sensitive and very efficient screening platform for direct measurements of ion transporters and ion channels in diverse and heterologous membranes. Since 2012, Nanion offers the SURFE2R product line in two formats: SURFE2R N1 and the higher throughput platform SURFE2R N96.
The SURFE2R N1 which we will present at the workshop is a small footprint, fully automated device recording from membrane preparations, with proven success using native tissue, mammalian and insect cell lines, bacteria, organelles, and proteoliposomes. Come to our workshop and learn from LIVE-experiments how to make measurements of transporter-protein functionality efficient and reliable!
Spaces are limited so reserve yours by sending an email to email@example.com.
Andrea Brüggemann, CSO, Nanion Technologies
Maria Barthmes, Student, Nanion Technologies
Molecular Devices, LLC
Tuesday, February 18, 1:00 PM - 2:30 PM
Axon Electrophysiology Symposium: Getting the Most out of pCLAMP Software
pCLAMP™ is a powerful data acquisition and analysis software and is widely used for a variety of electrophysiological recordings. In the first tutorial of this workshop, Jeffrey Tang will highlight a few features used to create a customized acquisition protocol in Clampex. In the second tutorial, Burt Maertz will share tips in single-channel analysis using Clampfit. These include burst analysis, latency analysis and P(open) analysis.
Jeffrey Tang, Product Marketing Manager, Axon Conventional Electrophysiology, Molecular Devices, LLC.
Burt Maertz, Technical Support Specialist, Axon Conventional Electrophysiology, Molecular Devices, LLC.
Tuesday, February 18, 3:00 PM - 4: 30 PM
The Devil is in the Detail: The Importance of Accurate Stability and Concentration Determination in Biomolecular Interaction Analysis
Stability of individual proteins and protein complexes are integral components of understanding molecular interactions. This workshop will focus on how differential scanning calorimetry (DSC) and surface plasmon resonance (SPR) can be used to paint a more complete picture of biomolecular interactions. Examples will be given showing the application of DSC to characterizing protein stability and the evolution of stability during drug development targeting multi-protein complexes.
A prerequisite to determining accurate affinities for a biomolecular interaction is knowledge of the active concentration of the binding partners. SPR is an exceptional technique for determining the active concentration of a protein and monitoring protein stability. Calibration-free concentration analysis (CFCA) utilizes principles of fluid mechanics and translational diffusion to yield the concentration of active protein without need for a standard. CFCA assays are easy to implement and technically robust. A case study will be shown illustrating that spectroscopic methods for determining concentration can yield inaccurate affinities, while SPR determinations of active protein concentration can be used to obtain accurate affinities and enhance understanding of properly folded protein.
Jason Schuman, Senior Label Free Interaction Analysis Application Scientist
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