A classical lay summary by Victoria Amari, from the University of Mississippi.
Cells constantly move and change shape to survive. Whether they’re healing a wound, growing new tissue, or responding to their environment, this motion depends on an internal support system called the cytoskeleton. Two key proteins in this system are actin and myosin. Actin forms long, rope-like filaments, while myosin acts like teams of people playing tug-of-war with that rope. By pulling on the actin “rope,” myosin generates force, causing the cell to stretch, contract, or move. Scientists have a good understanding of how a single myosin molecule pulls on actin, but much less is known about how large groups of myosin proteins work together. That raises big questions: Do they pull in sync? Does the actin rope stretch, resist, or even help guide the force? Our research investigates whether actin filaments act as more than just a passive rope—possibly working like smart sensors that influence how and when the myosin teams pull.
To study this, we use a technique called quartz crystal microbalance with dissipation monitoring (QCM-D). Imagine placing a vibrating plate under the game of tug-of-war. As people (myosin) pull on the rope (actin), the way the rope moves and stretches affects the vibrations. QCM-D is sensitive enough to detect tiny changes in weight and stiffness when proteins bind on top of a vibrating quartz surface. By analyzing these vibrations, we can see how actin and myosin work together under different conditions, like changing the number of myosin molecules or altering the energy available to them. Our early results suggest that actin does more than just get pulled—it may actually sense the tension and respond in ways that help coordinate myosin activity. This insight matters because when these mechanical interactions break down, it can lead to disease. For instance, in heart disease, improper contractions can result from faulty actin-myosin interactions. Even immune cells rely on this system to chase down infections. Understanding these forces at a molecular level could open new paths for treating diseases where cell movement goes awry.
QCM-D helps us measure the push-and-pull between actin and myosin, revealing how cells move, and how subtle mechanical signals inside the cell help maintain balance, or, when disrupted, lead to disease.